Genomic sequence capture of Plasmodium relictum in experimentally infected birds.

ABSTRACT:

Background

Sequencing parasite genomes in the presence of host DNA is challenging. Sequence capture can overcome this problem by using RNA probes that hybridize with the parasite DNA and then are removed from solution, thus isolating the parasite DNA for efficient sequencing.

Methods

Here we describe a set of sequence capture probes designed to target 1035 genes (c. 2.5 Mbp) of the globally distributed avian haemosporidian parasite, Plasmodium relictum. Previous sequence capture studies of avian haemosporidians from the genus Haemoproteus have shown that sequencing success depends on parasitemia, with low-intensity, chronic infections (typical of most infected birds in the wild) often being difficult to sequence. We evaluate the relationship between parasitemia and sequencin

SUBMITTER: Ellis VA 

PROVIDER: S-EPMC9336033 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature