Production of functional sperm from in vitro-cultured premeiotic spermatogonia in a marine fish.

ABSTRACT: In vitro production of functional gametes can revolutionize reproduction by reducing generation intervals and accelerating genetic breeding in aquaculture, especially in fish with relatively long generations. Nevertheless, functional sperm production from in vitro-cultured spermatogonia remains a challenge in most aquaculture fish. In this study, we isolated and characterized premeiotic spermatogonia from marine four-eyed sleepers ( Bostrychus sinensis), which are prone to ovotesticular or sterile testicular development, and induced the differentiation of the spermatogonia into flagellated sperm in a three-dimensional (3D) culture system. Artificial insemination indicated that the in vitro-derived sperm were capable of fertilizing mature oocytes to develop into

SUBMITTER: Zhang H 

PROVIDER: S-EPMC9336452 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature