Extending the capabilities of intact-mass analyses to monoclonal immunoglobulins of the E-isotype (IgE).

ABSTRACT: Mass spectrometry (MS) has become an indispensable tool in structural characterization and quality control of monoclonal antibodies (mAbs). Intact-mass analysis is a particularly attractive option that provides a powerful and cost-effective means to not only confirm the structural integrity of the protein, but also probe its interactions with therapeutic targets. To a certain extent, this success can be attributed to relatively modest glycosylation levels exhibited by IgG molecules, which limits their structural heterogeneity and enables straightforward mass measurements at the intact molecule level. The recent surge of interest in expanding the repertoire of mAbs to include other classes of immunoglobulins places a premium on efforts to adapt the IgG-tailored experimental strategies to ot

SUBMITTER: Yang W 

PROVIDER: S-EPMC9336480 | biostudies-literature | 2022 Jan-Dec

REPOSITORIES: biostudies-literature