Enriched circulating and tumor-resident TGF-β⁺ regulatory B cells in patients with melanoma promote FOXP3⁺ Tregs.

ABSTRACT: B cells are emerging as key players of anti-tumor adaptive immune responses. We investigated regulatory and pro-inflammatory cytokine-expressing B cells in patients with melanoma by flow cytometric intracellular cytokine, CyTOF, transcriptomic, immunofluorescence, single-cell RNA-seq, and B:T cell co-culture analyses. We found enhanced circulating regulatory (TGF-β⁺ and PD-L1⁺) and reduced pro-inflammatory TNF-α⁺ B cell populations in patients compared with healthy volunteers (HVs), including lower IFN-γ⁺:IL-4⁺ and higher TGF-β⁺:TNF-α⁺ B cell ratios in patients. TGF-β-expressing B cells in the melanoma tumor microenvironment assembled in clusters and interacted with T cells via lymphoid recruitment (SELL, CXCL13,

SUBMITTER: Harris RJ 

PROVIDER: S-EPMC9336482 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature