Human 2D Crypt Model for Assaying Intestinal Stem Cell Proliferation and Differentiation.

ABSTRACT: Intestine is a common site of adverse drug effects in clinical trials; thus, improved in vitro models for preclinical screening of pharmaceutical compounds are sought. A planar, self-renewing human intestinal monolayer platform based on primary adult gastrointestinal stem cells, termed the 2D crypt model, has been developed to screen for the effects of various compounds on the intestinal epithelium. The 2D crypt platform is based on a standard 12-well plate format and consists of cell culture inserts with a collagen film overlaying an impermeable film patterned with an array of micron-scale holes. This two-chamber format enables a gradient of growth factors to be applied such that the tissue self-organizes into spatially segregated stem and differentiated cell compartments. The patt

SUBMITTER: Wang Y 

PROVIDER: S-EPMC9337237 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature