Ontology highlight
ABSTRACT:
SUBMITTER: Yildirim M
PROVIDER: S-EPMC9337854 | biostudies-literature | 2022 Jul
REPOSITORIES: biostudies-literature
Yildirim Murat M Delepine Chloe C Feldman Danielle D Pham Vincent A VA Chou Stephanie S Ip Jacque J Nott Alexi A Tsai Li-Huei LH Ming Guo-Li GL So Peter T C PTC Sur Mriganka M
eLife 20220729
Human cerebral organoids are unique in their development of progenitor-rich zones akin to ventricular zones from which neuronal progenitors differentiate and migrate radially. Analyses of cerebral organoids thus far have been performed in sectioned tissue or in superficial layers due to their high scattering properties. Here, we demonstrate label-free three-photon imaging of whole, uncleared intact organoids (~2 mm depth) to assess early events of early human brain development. Optimizing a cust ...[more]