Landmark-based retrieval of inflamed skin vessels enabled by 3D correlative intravital light and volume electron microscopy.

ABSTRACT: The nanometer spatial resolution of electron microscopy imaging remains an advantage over light microscopy, but the restricted field of view that can be inspected and the inability to visualize dynamic cellular events are definitely drawbacks of standard transmission electron microscopy (TEM). Several methods have been developed to overcome these limitations, mainly by correlating the light microscopical image to the electron microscope with correlative light and electron microscopy (CLEM) techniques. Since there is more than one method to obtain the region of interest (ROI), the workflow must be adjusted according to the research question and biological material addressed. Here, we describe in detail the development of a three-dimensional CLEM workflow for mouse skin tissue exposed to an

SUBMITTER: Mildner K 

PROVIDER: S-EPMC9338004 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature