Functional neuronal circuitry and oscillatory dynamics in human brain organoids.

ABSTRACT: Human brain organoids replicate much of the cellular diversity and developmental anatomy of the human brain. However, the physiology of neuronal circuits within organoids remains under-explored. With high-density CMOS microelectrode arrays and shank electrodes, we captured spontaneous extracellular activity from brain organoids derived from human induced pluripotent stem cells. We inferred functional connectivity from spike timing, revealing a large number of weak connections within a skeleton of significantly fewer strong connections. A benzodiazepine increased the uniformity of firing patterns and decreased the relative fraction of weakly connected edges. Our analysis of the local field potential demonstrate that brain organoids contain neuronal assemblies of sufficient size and function

SUBMITTER: Sharf T 

PROVIDER: S-EPMC9338020 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature