A patient-designed tissue-engineered model of the infiltrative glioblastoma microenvironment.

ABSTRACT: Glioblastoma is an aggressive brain cancer characterized by diffuse infiltration. Infiltrated glioma cells persist in the brain post-resection where they interact with glial cells and experience interstitial fluid flow. We use patient-derived glioma stem cells and human glial cells (i.e., astrocytes and microglia) to create a four-component 3D model of this environment informed by resected patient tumors. We examine metrics for invasion, proliferation, and putative stemness in the context of glial cells, fluid forces, and chemotherapies. While the responses are heterogeneous across seven patient-derived lines, interstitial flow significantly increases glioma cell proliferation and stemness while glial cells affect invasion and stemness, potentially related to CCL2 expression and differenti

SUBMITTER: Cornelison RC 

PROVIDER: S-EPMC9338058 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature