New insights into sperm rheotaxis, agglutination and bundle formation in Sharkasi chickens based on an in vitro study.

ABSTRACT: Fertility in birds is dependent on their ability to store adequate populations of viable sperm for extended durations in sperm storage tubules (SSTs). The exact mechanisms by which sperm enter, reside, and egress from the SSTs are still controversial. Sharkasi chicken sperm showed a high tendency to agglutinate, forming motile thread-like bundles comprising many cells. Since it is difficult to observe sperm motility and behavior inside the opaque oviduct, we employed a microfluidic device with a microchannel cross-section resembling close to that of sperm glands allowing for the study of sperm agglutination and motility behavior. This study discusses how sperm bundles are formed, how they move, and what role they may have in extending sperm residency inside the SSTs. We investigated sperm

SUBMITTER: El-Sherry TM 

PROVIDER: S-EPMC9338266 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature