Thermoresponsive C22 phage stiffness modulates the phage infectivity.

ABSTRACT: Bacteriophages offer a sustainable alternative for controlling crop disease. However, the lack of knowledge on phage infection mechanisms makes phage-based biological control varying and ineffective. In this work, we interrogated the temperature dependence of the infection and thermo-responsive behavior of the C22 phage. This soilborne podovirus is capable of lysing Ralstonia solanacearum, causing bacterial wilt disease. We revealed that the C22 phage could better infect the pathogenic host cell when incubated at low temperatures (25, 30 °C) than at high temperatures (35, 40 °C). Measurement of the C22 phage stiffness revealed that the phage stiffness at low temperatures was 2-3 times larger than at high temperatures. In addition, the imaging results showed that more C22 phage particles we

SUBMITTER: Sae-Ueng U 

PROVIDER: S-EPMC9338302 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature