Investigation of urine metabolome of BALB/c mouse infected with an avirulent strain of Toxoplasma gondii.

ABSTRACT:

Background

The protozoan parasite Toxoplasma gondii is a major concern for human and animal health. Although the metabolic understanding of toxoplasmosis has increased in recent years, the analysis of metabolic alterations through noninvasive methodologies in biofluids remains limited.

Methods

Here, we applied liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based metabolomics and multivariate statistical analysis to analyze BALB/c mouse urine collected from acutely infected, chronically infected and control subjects.

Results

In total, we identified 2065 and 1409 metabolites in the positive electrospray ionization (ESI +) mode and ESI - mode, respectively. Metabolomic patterns generated from principal component analysis (PCA) and partial least squares discrim

SUBMITTER: Zhou CX 

PROVIDER: S-EPMC9338554 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature