Evidence for the circulating microRNA hsa-let-7d-3p as a potential new biomarker for sepsis in human subjects.

ABSTRACT:

Background

Current biomarkers for the early detection of sepsis have low sensitivity and specificity. Serum microRNAs (miRNAs) have been proposed as novel noninvasive biomarkers for various diseases. The aim of the present study was to discover a novel diagnostic biomarker for sepsis in human subjects.

Methods

miRNA expression profiling was performed using peripheral blood from three sepsis patients in the sepsis stage and improved condition stage using microarray screening. The differentially expressed miRNAs were primary validated by real-time quantitative polymerase chain reaction (RT-qPCR) in a further set of 20 sepsis patients in the sepsis stage and improved condition stage. Finally, we validated the differentially expressed miRNAs in 95 sepsis patients and 66 nonsepsi

SUBMITTER: Zhang Z 

PROVIDER: S-EPMC9338616 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature