Anterior cruciate ligament microfatigue damage detected by collagen autofluorescence in situ.

ABSTRACT:

Purpose

Certain types of repetitive sub-maximal knee loading cause microfatigue damage in the human anterior cruciate ligament (ACL) that can accumulate to produce macroscopic tissue failure. However, monitoring the progression of that ACL microfatigue damage as a function of loading cycles has not been reported. To explore the fatigue process, a confocal laser endomicroscope (CLEM) was employed to capture sub-micron resolution fluorescence images of the tissue in situ. The goal of this study was to quantify the in situ changes in ACL autofluorescence (AF) signal intensity and collagen microstructure as a function of the number of loading cycles.

Methods

Three paired and four single cadaveric knees were subjected to a repeated 4 times bodyweight landing maneuver known to str

SUBMITTER: Kim J 

PROVIDER: S-EPMC9339057 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature