Toward in vivo proof of binding of ¹⁸F-labeled inhibitor [¹⁸F]TRACK to peripheral tropomyosin receptor kinases.

ABSTRACT:

Background

Tropomyosin receptor kinases (TrkA, TrkB, TrkC) are a family of tyrosine kinases primarily expressed in neuronal cells of the brain. Identification of oncogenic alterations in Trk expression as a driver in multiple tumor types has increased interest in their role in human cancers. Recently, first- and second-generation ¹¹C and ¹⁸F-labeled Trk inhibitors, e.g., [¹⁸F]TRACK, have been developed. The goal of the present study was to analyze the direct interaction of [¹⁸F]TRACK with peripheral Trk receptors in vivo to prove its specificity for use as a functional imaging probe.

Methods

In vitro uptake and competition experiments were carried out using the colorectal cancer cell line KM12. Dynamic PET experiments were performe

SUBMITTER: Wuest M 

PROVIDER: S-EPMC9339071 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature