Aging Alters the Aortic Proteome in Health and Thoracic Aortic Aneurysm.

ABSTRACT:

Background

Aging enhances most chronic diseases but its impact on human aortic tissue in health and in thoracic aortic aneurysms (TAA) remains unclear.

Methods

We employed a human aortic biorepository of healthy specimens (n=17) and those that underwent surgical repair for TAA (n=20). First, we performed proteomics comparing aortas of healthy donors to aneurysmal specimens, in young (ie, <60 years of age) and old (ie, ≥60 years of age) subjects. Second, we measured proteins, via immunoblotting, involved in mitophagy (ie, Parkin) and also mitochondrial-induced inflammatory pathways, specifically TLR (toll-like receptor) 9, STING (stimulator of interferon genes), and IFN (interferon)-β.

Results

Proteomics revealed that aging transformed the aorta both quantitatively and

SUBMITTER: Tyrrell DJ 

PROVIDER: S-EPMC9339483 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature