Bacterial biofilm functionalization through Bap amyloid engineering.

ABSTRACT: Biofilm engineering has emerged as a controllable way to fabricate living structures with programmable functionalities. The amyloidogenic proteins comprising the biofilms can be engineered to create self-assembling extracellular functionalized surfaces. In this regard, facultative amyloids, which play a dual role in biofilm formation by acting as adhesins in their native conformation and as matrix scaffolds when they polymerize into amyloid-like fibrillar structures, are interesting candidates. Here, we report the use of the facultative amyloid-like Bap protein of Staphylococcus aureus as a tool to decorate the extracellular biofilm matrix or the bacterial cell surface with a battery of functional domains or proteins. We demonstrate that the localization of the functional tags can be chang

SUBMITTER: Matilla-Cuenca L 

PROVIDER: S-EPMC9339546 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature