Differential immune responses of 3D gingival and periodontal connective tissue equivalents to microbial colonization.

ABSTRACT: Gingival and periodontal ligament fibroblasts are functionally distinct cell types within the dento-gingival unit that participate in host immune response. Their microenvironment influences the behavior and immune response to microbial challenge. We developed three-dimensional gingival and periodontal connective tissue equivalents (CTEs) using human fibrin-based matrix. The CTEs were characterized, and the heterogeneity in their innate immune response was investigated. The CTEs demonstrated no to minimal response to planktonic Streptococcus mitis and Streptococcus oralis, while their biofilms elicited a moderate increase in IL-6 and IL-8 production. In contrast, Fusobacterium nucleatum provoked a substantial increase in IL-6 and IL-8 production. Interestingly, the ging

SUBMITTER: Makkar H 

PROVIDER: S-EPMC9340411 | biostudies-literature | 2022 Jan-Dec

REPOSITORIES: biostudies-literature