A Diaphanous and Enabled-dependent asymmetric actin cable array repositions nuclei during Drosophila oogenesis.

ABSTRACT: Cells reposition their nuclei for diverse specialized functions through a wide variety of cytoskeletal mechanisms. During Drosophila oogenesis, 15 nurse cells connected by ring canals to each other and the oocyte contract, 'dumping' their cytoplasm into the oocyte. Prior to dumping, actin cables initiate from the nurse cell cortex and elongate toward their nuclei, pushing them away from ring canals to prevent obstruction. How the cable arrays reposition nuclei is unknown. We found that these arrays are asymmetric, with regional differences in actin cable growth rate dependent on the differential localization of the actin assembly factors Enabled and Diaphanous. Enabled mislocalization produces a uniform growth rate. In oocyte-contacting nurse cells with asymmetric cable arrays, nuclei move

SUBMITTER: Logan G 

PROVIDER: S-EPMC9340552 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature