KIF2C affects sperm cell differentiation in patients with Klinefelter syndrome, as revealed by RNA-Seq and scRNA-Seq data.

ABSTRACT: Klinefelter syndrome (KS) is a leading contributor to male infertility and is characterised by complex and diverse clinical features; however, genetic changes in the KS transcriptome remain largely unknown. We therefore used transcriptomic and single-cell RNA sequencing (scRNA-seq) datasets from KS versus normal populations through the Gene Expression Omnibus (GEO) database to identify gene biomarkers associated with the occurrence of KS. We identified a total of 700 differentially expressed genes (DEGs) and completed Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), enrichment pathway analysis and protein-protein interaction (PPI) network analysis. A total of four unreported KS-related hub genes (KIF2C, MRPS2, RPS15 and TSFM) were identified. Validation of the single-cel

SUBMITTER: He H 

PROVIDER: S-EPMC9340869 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature