Association between ANXA5 haplotypes and the risk of recurrent pregnancy loss.

ABSTRACT:

Background

Annexin A5 (ANXA5) haplotypes can increase the risk of recurrent pregnancy loss (RPL). This study aimed to investigate the effect of ANXA5 haplotypes on ANXA5 expression in patients with RPL.

Methods

Female subjects with RPL, parous controls (those who intentionally aborted without medical conditions or complications), and population controls (normal delivery) were studied. Real-time polymerase chain reaction was carried out to evaluate ANXA5 expression in the placenta and peripheral blood. Western blotting and immunohistochemistry were used to assess ANXA5 protein expression. The luciferase assay was performed to detect the effect of M1 and M2 haplotypes on transcription efficiency of the ANXA5 promoter.

Results

We found that the percentage of the M2 carri

SUBMITTER: Zheng M 

PROVIDER: S-EPMC9340999 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature