Comparison of gdh polymerase chain reaction-restriction fragment length polymorphism and tpi assemblage-specific primers for characterization of Giardia intestinalis in children.

ABSTRACT:

Background

Giardia is a diarrheagenic eukaryotic parasite that consists of at least eight morphologically identical but genetically distinct genotypes. Human giardiasis is caused mainly by A and B assemblages.

Aim and objectives

The study aimed to compare the performance of gdh polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and tpi assemblage-specific primers in genotyping of G. intestinalis.

Materials and methods

Stool samples of 315 children were microscopically screened for G. intestinalis. Positive samples were genotyped using tpi assemblage-specific primers and gdh semi-nested PCR-RFLP techniques.

Results

The prevalence of Giardia was 18.1%. The detected genotypes using

SUBMITTER: Elhadad H 

PROVIDER: S-EPMC9341145 | biostudies-literature | 2022 Jan-Jun

REPOSITORIES: biostudies-literature