AAMP is a binding partner of costimulatory human B7-H3.

ABSTRACT:

Background

Targeted immunotherapies are of growing interest in the treatment of various cancers. B7 homolog 3 protein (B7-H3), a member of the co-stimulatory/-inhibitory B7-family, exerts immunosuppressive and pro-tumorigenic functions in various cancer types and is under evaluation in ongoing clinical trials. Unfortunately, interaction partner(s) remain unknown which restricts the druggability.

Methods

Aiming to identify potential binding partner(s) of B7-H3, a yeast two-hybrid and a mass spectrometry screen were performed. Potential candidates were evaluated by bimolecular fluorescence complementation (BiFC) assay, co-immunoprecipitation (co-IP), and functionally in a ³H-thymidine proliferation assay of Jurkat cells, a T-cell lineage cell line. Prognostic value

SUBMITTER: Ciprut S 

PROVIDER: S-EPMC9341442 | biostudies-literature | 2022 Jan-Dec

REPOSITORIES: biostudies-literature