Overexpression of cytoplasmic C₄ Flaveria bidentis carbonic anhydrase in C₃ Arabidopsis thaliana increases amino acids, photosynthetic potential, and biomass.

ABSTRACT: An important method to improve photosynthesis in C₃ crops, such as rice and wheat, is to transfer efficient C₄ characters to them. Here, cytosolic carbonic anhydrase (CA: βCA3) of the C₄ Flaveria bidentis (Fb) was overexpressed under the control of ³⁵ S promoter in Arabidopsis thaliana, a C₃ plant, to enhance its photosynthetic efficiency. Overexpression of CA resulted in a better supply of the substrate HCO3- for the endogenous phosphoenolpyruvate carboxylase in the cytosol of the overexpressers, and increased its activity for generating malate that feeds into the tricarboxylic acid cycle. This provided additional carbon skeleton for increased synthesis of amino acids aspartate, asparagine, glutamate, and glutamine. Increased amino ac

SUBMITTER: Kandoi D 

PROVIDER: S-EPMC9342616 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature