Dataset Information

JULGI-mediated increment in phloem transport capacity relates to fruit yield in tomato.

ABSTRACT: The continuous growth of the global population and the increase in the amount of arid land has severely constrained agricultural crop production. To solve this problem, many researchers have attempted to increase productivity through the efficient distribution of energy; however, the direct relationship between the plant vasculature, specifically phloem development, and crop yield is not well established. Here, we demonstrate that an optimum increase in phloem-transportation capacity by reducing SIJUL expression leads to improved sink strength in tomato (Solanum lycopersicum L.). SIJUL, a negative regulator of phloem development, suppresses the translation of a positive regulator of phloem development, SlSMXL5. The suppression of SlJUL increases the number of phloem cells and sucrose transport, but only an optimal reduction of SlJUL function greatly enhances sink strength in tomato, improving fruit setting, and yield contents by 37% and 60%, respectively. We show that the increment in phloem cell number confers spare transport capacity. Our results suggest that the control of phloem-transport capacity within the threshold could enhance the commitment of photosynthates to instigate yield improvement.

SUBMITTER: Nam H

PROVIDER: S-EPMC9342617 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature

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JULGI-mediated increment in phloem transport capacity relates to fruit yield in tomato.

Nam Hoyoung H Gupta Aarti A Nam Heejae H Lee Seungchul S Cho Hyun Seob HS Park Chanyoung C Park Soyoung S Park Soon Ju SJ Hwang Ildoo I

Plant biotechnology journal 20220517 8

The continuous growth of the global population and the increase in the amount of arid land has severely constrained agricultural crop production. To solve this problem, many researchers have attempted to increase productivity through the efficient distribution of energy; however, the direct relationship between the plant vasculature, specifically phloem development, and crop yield is not well established. Here, we demonstrate that an optimum increase in phloem-transportation capacity by reducing ...[more]

PMID: 35478430

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Project description:Genetic modification of Cry-proteins from Bacillus thuringiensis (Bt) is a common practice in economically important crops to improve insecticide resistance and reduce the use of pesticides. However, introduction of these genes can have unintended side effects, which should be closely monitored for effective breeding and crop management. To determine the potential cause of these negative effects, we explored assimilate partitioning in the transgenic Bt rice line T1c-19 (Cry1C*), which was compared with that of its wild-type counterpart Minghui 63 (MH63) under different potassium fertilization application treatment conditions. In a pot experiment, 0, 0.4, and 0.6 g K2O was applied per kg of dry soil to determine the phloem transport characteristics of the two rice lines. We used a variety of assessment indicators ranging from morphological to physiological aspects, including the number of large and small vascular bundles in the neck internode at the heading stage, the diameter and bleeding intensity of the neck internode at the filling stage, and the content and apparent ratio of transferred non-structural carbohydrates (NSC) in the culm and sheath from the heading to maturing stages. The K utilization and grain yield at the maturing stage were also concerned. Results presented that the mean setting rate and grain yield of T1c-19 (Cry1C*) decreased by 22.3% and 26.2% compared to those in MH63, respectively. Compared to MH63, the K concentration and accumulation were significantly higher in the culms and leaves, but significantly lower in grain of T1c-19 (Cry1C*). T1c-19 (Cry1C*) had less apparent NSC efflux in the culm and sheath, fewer small vascular bundles, and a smaller diameter and bleeding intensity of the neck internode than MH63. In addition, linear correlation analysis indicated that there were positive correlations among grain yield, setting rate, the apparent NSC efflux in the culm and sheath, number of small vascular bundles, and the neck internode diameter and bleeding intensity. These unintended effects may directly or indirectly be caused by insertion of exogenous Bt (Cry1C*) gene, which should be further considered in the future breeding of transgenic crops.

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Project description:Salt stress generally induces important negative effects on tomato (Solanum lycopersicum) productivity but it may also cause a positive effect improving fruit quality, one of the greatest challenges in nowadays agriculture. Because of the genetic erosion of this horticultural species, the recovery of locally adapted landraces could play a very important role in avoiding, at least partially, production losses and simultaneously improving fruit quality. Two tomato landraces endemic of the Spanish Southeast area, characterized by the harsh climatic conditions of the Mediterranean basin, have been selected: Negro Yeste (NY) characterized by its dark-red colored fruits and Verdal (V), which fruits did not achieve the characteristic red color at ripening. Here the agronomic, physiological, and metabolic responses of these landraces were compared with the reference tomato commercial cv. Moneymaker (MM), in plants grown without salt (control) and with salt stress (100 mM NaCl) for 70 days. The higher salt tolerance of both landraces was mainly reflected in the fruit number, as NY only reduced the fruit number in salt stress by 20% whereas in MM it was reduced till 43%, and in V the fruit number even showed an increase of 33% with salt stress. An important fruit quality parameter is soluble solids content, which increases induced by salinity were significantly higher in both landraces (60 and 78% in NY and V, respectively) compared with MM (34%). Although both landraces showed a similar response in relation to the high chlorophyll accumulation detected in their fruits, the fruit metabolic profiles were very different. Increased carotenoids levels were found in NY fruits, especially lycopene in ripe fruit, and this characteristic was observed in both control and salt stress. Contrarily, the carotenoid biosynthesis pathway was disrupted in V ripe fruits, but other metabolites, such as Ca2+, mannose, formate, and glutamate were accumulated. These results highlight the potential of tomato landraces to improve nutritional fruit quality and maintain fruit yield stability under salt stress.

Dataset Information

JULGI-mediated increment in phloem transport capacity relates to fruit yield in tomato.

Publications

JULGI-mediated increment in phloem transport capacity relates to fruit yield in tomato.

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