Renal Deletion of LRRC8/VRAC Channels Induces Proximal Tubulopathy.

ABSTRACT:

Background

Volume-regulated anion channels (VRACs) are heterohexamers of LRRC8A with LRRC8B, -C, -D, or -E in various combinations. Depending on the subunit composition, these swelling-activated channels conduct chloride, amino acids, organic osmolytes, and drugs. Despite VRACs' role in cell volume regulation, and large osmolarity changes in the kidney, neither the localization nor the function of VRACs in the kidney is known.

Methods

Mice expressing epitope-tagged LRRC8 subunits were used to determine the renal localization of all VRAC subunits. Mice carrying constitutive deletions of Lrrc8b-e, or with inducible or cell-specific ablation of Lrrc8a, were analyzed to assess renal functions of VRACs. Analysis included histology, urine and serum parameters

SUBMITTER: Lopez-Cayuqueo KI 

PROVIDER: S-EPMC9342636 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature