An autologous colonic organoid‐derived monolayer model to study immune: bacterial interactions in Crohn's disease patients

ABSTRACT: Abstract

Objectives

Crohn's disease (CD) initiation and pathogenesis are believed to involve an environmental trigger in a genetically susceptible person that results in an immune response against commensal gut bacteria, leading to a compromised intestinal epithelial barrier and a cycle of inflammation. However, it has been difficult to study the contribution of all factors together in a physiologically relevant model and in a heterogenous patient population.

Methods

We developed an autologous colonic monolayer model that incorporated the immune response from the same donor and a commensal bacteria, Faecalibacterium prausnitzii. Two‐dimensional monolayers were grown from three‐dimensional organoids generated from intestinal biopsies, and the epithelial integrity of the ep

SUBMITTER: Angus H 

PROVIDER: S-EPMC9342672 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature