Directing cyanobacterial photosynthesis in a cytochrome c oxidase mutant using a heterologous electron sink.

ABSTRACT: Photosynthesis holds the promise of sustainable generation of useful products using light energy. Key to realizing this potential is the ability to rationally design photosynthesis to redirect energy and reductant derived from photons to desired products. Cytochrome P450s (P450s), which catalyze a broad array of reactions, have been engineered into a variety of photosynthetic organisms, where their activity has been shown to be photosynthesis-dependent, thus acting as heterologous sinks of electrons derived from photosynthesis. Furthermore, the addition of P450s can increase the photosynthetic capacity of the host organism. In this study, we developed this technology further using a P450 (CYP1A1) expressed in the cyanobacterium Synechococcus sp. PCC 7002. We show that rationally engineerin

SUBMITTER: Torrado A 

PROVIDER: S-EPMC9342982 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature