High-content imaging of human hepatic spheroids for researching the mechanism of duloxetine-induced hepatotoxicity.

ABSTRACT: Duloxetine (DLX) has been approved for the successful treatment of psychiatric diseases, including major depressive disorder, diabetic neuropathy, fibromyalgia and generalized anxiety disorder. However, since the usage of DLX carries a manufacturer warning of hepatotoxicity given its implication in numerous cases of drug-induced liver injuries (DILI), it is not recommended for patients with chronic liver diseases. In our previous study, we developed an enhanced human-simulated hepatic spheroid (EHS) imaging model system for performing drug hepatotoxicity evaluation using the human hepatoma cell line HepaRG and the support of a pulverized liver biomatrix scaffold, which demonstrated much improved hepatic-specific functions. In the current study, we were able to use this robust model to demo

SUBMITTER: Liu J 

PROVIDER: S-EPMC9343405 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature