CTCF organizes inter-A compartment interactions through RYBP-dependent phase separation.

ABSTRACT: Chromatin is spatially organized into three-dimensional structures at different levels including A/B compartments, topologically associating domains and loops. The canonical CTCF-mediated loop extrusion model can explain the formation of loops. However, the organization mechanisms underlying long-range chromatin interactions such as interactions between A-A compartments are still poorly understood. Here we show that different from the canonical loop extrusion model, RYBP-mediated phase separation of CTCF organizes inter-A compartment interactions. Based on this model, we designed and verified an induced CTCF phase separation system in embryonic stem cells (ESCs), which facilitated inter-A compartment interactions, improved self-renewal of ESCs and inhibited their differentiation toward neu

SUBMITTER: Wei C 

PROVIDER: S-EPMC9343660 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature