Single-Cell Transcriptomics Reveals Killing Mechanisms of Antitumor Cytotoxic CD4⁺ TCR-T Cells.

ABSTRACT: T cell receptor-engineered T cells (TCR-Ts) have emerged as potent cancer immunotherapies. While most research focused on classical cytotoxic CD8⁺ T cells, the application of CD4⁺ T cells in adoptive T cell therapy has gained much interest recently. However, the cytotoxic mechanisms of CD4⁺ TCR-Ts have not been fully revealed. In this study, we obtained an MHC class I-restricted MART-1_27-35-specific TCR sequence based on the single-cell V(D)J sequencing technology, and constructed MART-1_27-35-specific CD4⁺ TCR-Ts and CD8⁺ TCR-Ts. The antitumor effects of CD4⁺ TCR-Ts were comparable to those of CD8⁺ TCR-Ts in vitro and in vivo. To delineate the killing mechanisms of cytotoxic CD4

SUBMITTER: Liang Y 

PROVIDER: S-EPMC9343810 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature