A four-dimensional view of assembly of a morphogenetic protein during sporulation in Bacillus subtilis.

ABSTRACT: We report the use of a fusion to the green fluorescent protein to visualize the assembly of the morphogenetic protein SpoIVA around the developing forespore during the process of sporulation in the bacterium Bacillus subtilis. Using a deconvolution algorithm to process digitally-collected optical sections, we show that SpoIVA, which is synthesized in the mother cell chamber of the sporangium, assembled into a spherical shell around the outer surface of the forespore. Time-lapse fluorescence microscopy showed that this assembly process commenced at the time of polar division and seemed to continue after engulfment of the forespore was complete. SpoIVA remained present throughout the late stages of morphogenesis and was present as a component of the fully mature spore. Evidence indicates tha

SUBMITTER: Price KD 

PROVIDER: S-EPMC93443 | biostudies-literature | 1999 Feb

REPOSITORIES: biostudies-literature