Efficient production of clerodane and ent-kaurane diterpenes through truncated artificial pathways in Escherichia coli.

ABSTRACT: The clerodane and ent-kaurane diterpenoids are two typical categories of diterpenoid natural products with complicated polycyclic carbon skeletons and significant pharmacological activities. Despite exciting advances in organic chemistry, access to these skeletons is still highly challenging. Using synthetic biology to engineer microbes provides an innovative alternative to bypass synthetic challenges. In this study, we constructed two truncated artificial pathways to efficiently produce terpentetriene and ent-kaurene, two representative clerodane and ent-kaurane diterpenes, in Escherichia coli. Both pathways depend on the exogenous addition of isoprenoid alcohol to reinforce the supply of IPP and DMAPP via two sequential phosphorylation reactions. Optimization

SUBMITTER: Li FR 

PROVIDER: S-EPMC9344551 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature