Iron controls T helper cell pathogenicity by promoting glucose metabolism in autoimmune myopathy.

ABSTRACT:

Background

T helper cells in patients with autoimmune disease of idiopathic inflammatory myopathies (IIM) are characterized with the proinflammatory phenotypes. The underlying mechanisms remain unknown.

Methods

RNA sequencing was performed for differential expression genes. Gene expression in CD4⁺ T-cells was confirmed by quantitative real-time PCR. CD4⁺ T-cells from IIM patients or healthy controls were evaluated for metabolic activities by Seahorse assay. Glucose uptake, T-cell proliferation and differentiation were evaluated and measured by flow cytometry. Human CD4⁺ T-cells treated with iron chelators or Pfkfb4 siRNA were measured for glucose metabolism, proliferation and differentiation. Signalling pathway activation was evaluated by wes

SUBMITTER: Lai Y 

PROVIDER: S-EPMC9345506 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature