Determination of oligosaccharide product distributions of PL7 alginate lyases by their structural elements.

ABSTRACT: Alginate lyases can be used to produce well-defined alginate oligosaccharides (AOSs) because of their specificities for AOS products. A large number of alginate lyases have been recorded in the CAZy database; however, the majority are annotated-only alginate lyases that include little information on their products, thus limiting their applications. Here, we establish a simple and experiment-saving approach to predict product distributions for PL7 alginate lyases through extensive structural biology, bioinformatics and biochemical studies. Structural study on several PL7 alginate lyases reveals that two loops around the substrate binding cleft determine product distribution. Furthermore, a database containing the loop information of all annotated-only single-domain PL7 alginate lyases is co

SUBMITTER: Zhang K 

PROVIDER: S-EPMC9345997 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature