Digital quantification of somatostatin receptor subtype 2a immunostaining: a validation study.

ABSTRACT:

Objective

The aim of this study was to develop an open-source and reproducible digital quantitative analysis (DIA) of somatostatin receptor subtype 2a (SST2) staining in formalin-fixed paraffin-embedded tissues of pancreatic neuroendocrine tumors (panNETs) and growth hormone (GH)-secreting pituitary adenomas (GHomas).

Design

SST2 immunostaining of 18 panNETs and 39 GHomas was assessed using a novel DIA protocol and compared with a widely used semi-quantitative immunoreactivity score (IRS).

Methods

The DIA software calculates the staining intensity/area and the percentage of positive cells (%PC). Four representative images were selected for each sample by two independent selectors (S1 and S2), with the analysis performed by two independent analyzers (A1 and A2). Agreem

SUBMITTER: Campana C 

PROVIDER: S-EPMC9346267 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature