Identification of a conservative site in the African swine fever virus p54 protein and its preliminary application in a serological assay.

ABSTRACT:

Background

ASF was first reported in Kenya in 1910 in 1921. In China, ASF spread to 31 provinces including Henan and Jiangsu within six months after it was first reported on August 3, 2018. The epidemic almost affected the whole China, causing direct economic losses of tens of billions of yuan. Cause great loss to our pig industry. As ELISA is cheap and easy to operate, OIE regards it as the preferred serological method for ASF detection. P54 protein has good antigenicity and is an ideal antigen for detection.

Objective

To identify a conservative site in the African swine fever virus (ASFV) p54 protein and perform a Cloth-enzyme-linked immunosorbent assay (ELISA) for detecting the ASFV antibody in order to reduce risks posed by using the live virus in diagnostic assays.

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SUBMITTER: Xu L 

PROVIDER: S-EPMC9346533 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature