Effect of tumor necrosis factor alpha (TNF-α) -308 and -1031 gene polymorphisms on periodontitis among Saudi subjects.

ABSTRACT:

Objectives

Periodontitis is an infectious disorder that leads to irreversible loss of the surrounding attachment and bone destruction. Genetic polymorphism of cytokines has been suggested to play a role in periodontitis. This case-control study aimed to investigate the relationship between periodontitis and two single nucleotide polymorphisms (SNPs): rs1800629 (-308 G/A) and rs1799964 (-1031 T/C), in the TNF-α gene promoter area.

Materials and methods

Peripheral blood was used to prepare genomic DNA from 60 subjects with stage II to stage III periodontitis, as along with 65 control subjects. Polymerase chain reaction and restriction endonuclease digestion were used to genotype TNF-α SNPs.

Results

The distribution of both genotypes and alleles of TNF-α (-308 G/A) polym

SUBMITTER: Azab E 

PROVIDER: S-EPMC9346946 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature