Structural basis of NPR1 in activating plant immunity.

ABSTRACT: NPR1 is a master regulator of the defence transcriptome induced by the plant immune signal salicylic acid^1-4. Despite the important role of NPR1 in plant immunity^5-7, understanding of its regulatory mechanisms has been hindered by a lack of structural information. Here we report cryo-electron microscopy and crystal structures of Arabidopsis NPR1 and its complex with the transcription factor TGA3. Cryo-electron microscopy analysis reveals that NPR1 is a bird-shaped homodimer comprising a central Broad-complex, Tramtrack and Bric-à-brac (BTB) domain, a BTB and carboxyterminal Kelch helix bundle, four ankyrin repeats and a disordered salicylic-acid-binding domain. Crystal structure analysis reveals a unique zinc-finger motif in BTB for interacting with ankyrin repeats an

SUBMITTER: Kumar S 

PROVIDER: S-EPMC9346951 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature