Mutant polymerases capable of 2' fluoro-modified nucleic acid synthesis and amplification with improved accuracy.

ABSTRACT: Nonnatural nucleic acids (xeno nucleic acids, XNA) can possess several useful properties such as expanded reactivity and nuclease resistance, which can enhance the utility of DNA as a biotechnological tool. Native DNA polymerases are unable to synthesize XNA, so, in recent years mutant XNA polymerases have been engineered with sufficient activity for use in processes such as PCR. While substantial improvements have been made, accuracy still needs to be increased by orders of magnitude to approach natural error rates and make XNA polymerases useful for applications that require high fidelity. Here, we systematically evaluate leading Taq DNA polymerase mutants for their fidelity during synthesis of 2'F XNA. To further improve their accuracy, we add mutations that have been shown to increase

SUBMITTER: Christensen TA 

PROVIDER: S-EPMC9347352 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature