Knockdown of Transmembrane Protein 150A (TMEM150A) Results in Increased Production of Multiple Cytokines.

ABSTRACT: Lipopolysaccharide (LPS)-induced signaling through Toll-like receptor 4 (TLR4) is mediated by the plasma membrane lipid, phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P₂] and its derivatives diacylglycerol and inositol trisphosphate. Levels of PI(4,5)P₂ are controlled enzymatically and fluctuate in LPS-stimulated cells. Recently, transmembrane protein 150A (TMEM150A/TM6P1/damage-regulated autophagy modulator 5) has been shown to regulate PI(4,5)P₂ production at the plasma membrane by modifying the composition of the phosphatidylinositol 4-kinase enzyme complex. To determine if TMEM150A function impacts TLR4 signaling, TMEM150A was knocked down in TLR4-expressing epithelial cells and cytokine expression quantified after LPS stimulation. In general, decreas

SUBMITTER: Romanet JL 

PROVIDER: S-EPMC9347386 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature