Low molecular weight chitooligosaccharide inhibits infection of SARS-CoV-2 in vitro.

ABSTRACT:

Aims

The discovery of antiviral substances to respond to COVID-19 is a global issue, including the field of drug development based on natural materials. Here, we showed that chitosan-based substances have natural antiviral properties against SARS-CoV-2 in vitro.

Methods and results

The molecular weight of chitosan-based substances was measured by the gel permeation chromatography analysis. In MTT assay, the chitosan-based substances have low cytotoxicity to Vero cells. The antiviral effect of these substances was confirmed by quantitative viral RNA targeting the RdRp and E genes and plaque assay. Among the substances tested, low molecular weight chitooligosaccharide decreased the fluorescence intensity of SARS-CoV-2 nucleocapsid protein of the virus-infected cells in a dose-

SUBMITTER: Jang D 

PROVIDER: S-EPMC9347542 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature