Epitope Profiling Reveals the Antibody Immune Response Difference Between COVID‐19 Infected and Vaccinated

ABSTRACT: Antibodies play an important part in combating SARS‐CoV‐2 infection whether generated by the infection or vaccination. However, the many epitopes generated by infection have not been fully investigated with only a few epitopes known and these being mostly limited to the S protein’s receptor binding domain (RBD) and the N‐terminal domain which limits vaccine and drug design 1‐4. The difference between epitopes generated by infection and vaccination has also not been studied. To address this, we employed a SARS‐CoV‐2 proteome microarray to screen for linear epitopes recognized by antibodies present in COVID‐19 patients and individuals vaccinated with the Pfizer‐BioNTech mRNA COVID‐19 Vaccine. The proteome microarray consisted of S, N, and E proteins, as well as spotting peptides that were 15

SUBMITTER: Pierson L 

PROVIDER: S-EPMC9347619 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature