A 3D-Printed Customizable Platform for Multiplex Dynamic Biofilm Studies.

ABSTRACT: Biofilms are communities of microbes that colonize surfaces. While several biofilm experimental models exist, they often have limited replications of spatiotemporal dynamics surrounding biofilms. For a better understanding dynamic and complex biofilm development, this manuscript presents a customizable platform compatible with off-the-shelf well plates that can monitor microbial adhesion, growth, and associated parameters under various relevant scenarios by taking advantage of 3D printing. The system i) holds any substrate in a stable, vertical position, ii) subjects samples to flow at different angles, iii) switches between static and dynamic modes during an experiment, and iv) allows multiplexing and real-time monitoring of biofilm parameters. Simulated fluid dynamics is employed to esti

SUBMITTER: Dhall A 

PROVIDER: S-EPMC9347831 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature