Dataset Information

Glioblastoma-mediated Immune Dysfunction Limits CMV-specific T Cells and Therapeutic Responses: Results from a Phase I/II Trial.

ABSTRACT:

Purpose

Cytomegalovirus (CMV) antigens occur in glioblastoma but not in normal brains, making them desirable immunologic targets.

Patients and methods

Highly functional autologous polyclonal CMV pp65-specific T cells from patients with glioblastoma were numerically expanded under good manufacturing practice compliant conditions and administered after 3 weeks of lymphodepleting dose-dense temozolomide (100 mg/m²) treatment. The phase I component used a 3+3 design, ascending through four dose levels (5 × 10⁶-1 × 10⁸ cells). Treatment occurred every 6 weeks for four cycles. In vivo persistence and effector function of CMV-specific T cells was determined by dextramer staining and multiparameter flow cytometry in serially sampled peripheral blood and in the tumor microenvironment.

Results

We screened 65 patients; 41 were seropositive for CMV; 25 underwent leukapheresis; and 20 completed ≥1 cycle. No dose-limiting toxicities were observed. Radiographic response was complete in 1 patient, partial in 2. Median progression-free survival (PFS) time was 1.3 months [95% confidence interval (CI), 0-8.3 months]; 6-month PFS was 19% (95% CI, 7%-52%); and median overall survival time was 12 months (95% CI, 6 months to not reached). Repeated infusions of CMV-T cells paralleled significant increases in circulating CMV⁺ CD8⁺ T cells, but cytokine production showing effector activity was suppressed, especially from T cells obtained directly from glioblastomas.

Conclusions

Adoptive infusion of CMV-specific T cells after lymphodepletion with dose-dense temozolomide was well tolerated. But apparently CMV seropositivity does not guarantee tumor susceptibility to CMV-specific T cells, suggesting heterogeneity in CMV antigen expression. Moreover, effector function of these T cells was attenuated, indicating a requirement for further T-cell modulation to prevent their dysfunction before conducting large-scale clinical studies.

SUBMITTER: Weathers SP

PROVIDER: S-EPMC9347965 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature

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Publications

Glioblastoma-mediated Immune Dysfunction Limits CMV-specific T Cells and Therapeutic Responses: Results from a Phase I/II Trial.

Weathers Shiao-Pei SP Penas-Prado Marta M Pei Be-Lian BL Ling Xiaoyang X Kassab Cynthia C Banerjee Pinaki P Bdiwi Mustafa M Shaim Hila H Alsuliman Abdullah A Shanley Mayra M de Groot John F JF O'Brien Barbara J BJ Harrison Rebecca R Majd Nazanin N Kamiya-Matsuoka Carlos C Fuller Gregory N GN Huse Jason T JT Chi Linda L Rao Ganesh G Weinberg Jeffrey S JS Lang Frederick F FF Sawaya Raymond R Shpall Elizabeth J EJ Rezvani Katayoun K Heimberger Amy B AB

Clinical cancer research : an official journal of the American Association for Cancer Research 20200416 14

<h4>Purpose</h4>Cytomegalovirus (CMV) antigens occur in glioblastoma but not in normal brains, making them desirable immunologic targets.<h4>Patients and methods</h4>Highly functional autologous polyclonal CMV pp65-specific T cells from patients with glioblastoma were numerically expanded under good manufacturing practice compliant conditions and administered after 3 weeks of lymphodepleting dose-dense temozolomide (100 mg/m<sup>2</sup>) treatment. The phase I component used a 3+3 design, ascend ...[more]

PMID: 32299815

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Dataset Information

Glioblastoma-mediated Immune Dysfunction Limits CMV-specific T Cells and Therapeutic Responses: Results from a Phase I/II Trial.

Purpose

Patients and methods

Results

Conclusions

Publications

Glioblastoma-mediated Immune Dysfunction Limits CMV-specific T Cells and Therapeutic Responses: Results from a Phase I/II Trial.

Similar Datasets

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