Maternal H3K36 and H3K27 HMTs protect germline development via regulation of the transcription factor LIN-15B.

ABSTRACT: Maternally synthesized products play critical roles in the development of offspring. A premier example is the Caenorhabditis elegans H3K36 methyltransferase MES-4, which is essential for germline survival and development in offspring. How maternal MES-4 protects the germline is not well understood, but its role in H3K36 methylation hinted that it may regulate gene expression in primordial germ cells (PGCs). We tested this hypothesis by profiling transcripts from nascent germlines (PGCs and their descendants) dissected from wild-type and mes-4 mutant (lacking maternal and zygotic MES-4) larvae. mes-4 nascent germlines displayed downregulation of some germline genes, upregulation of some somatic genes, and dramatic upregulation of hundreds of genes on the X chromosome. W

SUBMITTER: Cockrum CS 

PROVIDER: S-EPMC9348848 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature