Claudin5 protects the peripheral endothelial barrier in an organ and vessel-type-specific manner.

ABSTRACT: Dysfunctional and leaky blood vessels resulting from disruption of the endothelial cell (EC) barrier accompanies numerous diseases. The EC barrier is established through endothelial cell tight and adherens junctions. However, the expression pattern and precise contribution of different junctional proteins to the EC barrier is poorly understood. Here, we focus on organs with continuous endothelium to identify structural and functional in vivo characteristics of the EC barrier. Assembly of multiple single-cell RNAseq datasets into a single integrated database revealed the variability and commonalities of EC barrier patterning. Across tissues, Claudin5 exhibited diminishing expression along the arteriovenous axis, correlating with EC barrier integrity. Functional analysis identified tissue-sp

SUBMITTER: Richards M 

PROVIDER: S-EPMC9348850 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature