Erwinia tasmaniensis levansucrase shows enantiomer selection for (S)-1,2,4-butanetriol.

ABSTRACT: Levansucrases are biotechnologically interesting fructosyltransferases due to their potential use in the enzymatic or chemo-enzymatic synthesis of glycosides of non-natural substrates relevant to pharmaceutical applications. The structure of Erwinia tasmaniensis levansucrase in complex with (S)-1,2,4-butanetriol and its biochemical characterization suggests the possible application of short aliphatic moieties containing polyols with defined stereocentres in fructosylation biotechnology. The structural information revealed that (S)-1,2,4-butanetriol mimics the natural substrate. The preference of the protein towards a specific 1,2,4-butanetriol enantiomer was assessed using microscale thermophoresis binding assays. Furthermore, the results obtained and the structural comparison of levansucr

SUBMITTER: Polsinelli I 

PROVIDER: S-EPMC9350837 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature