A novel lytic phage potentially effective for phage therapy against Burkholderia pseudomallei in the tropics.

ABSTRACT:

Background

Burkholderia pseudomallei is a tropical pathogen that causes melioidosis. Its intrinsic drug-resistance is a leading cause of treatment failure, and the few available antibiotics require prolonged use to be effective. This study aimed to assess the clinical potential of B. pseudomallei phages isolated from Hainan, China.

Methods

Burkholderia pseudomallei strain (HNBP001) was used as the isolation host, and phages were recovered from domestic environmental sources, which were submitted to the host range determination, lytic property assays, and stability tests. The best candidate was examined via the transmission electron microscope for classification. With its genome sequenced and analyzed, its protective efficacy against B. pseudomallei infection in A549 cells an

SUBMITTER: Wang Y 

PROVIDER: S-EPMC9351088 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature