RNA sequencing reveals dynamic expression of spleen lncRNAs and mRNAs in Beagle dogs infected by Toxocara canis.

ABSTRACT:

Background

Toxocara canis is a cosmopolitan parasite with a significant adverse impact on the health of humans and animals. The spleen is a major immune organ that plays essential roles in protecting the host against various infections. However, its role in T. canis infection has not received much attention.

Methods

We performed sequencing-based transcriptome profiling of long noncoding RNA (lncRNA) and messenger RNA (mRNA) expression in the spleen of Beagle puppies at 24 h post-infection (hpi), 96 hpi and 36 days post-infection (dpi). Deep sequencing of RNAs isolated from the spleen of six puppies (three infected and three control) at each time point after infection was conducted.

Results

Our analysis revealed 614 differentially expressed (DE) lncRNAs and 262 DEmRNAs

SUBMITTER: Xu Y 

PROVIDER: S-EPMC9351231 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature